EXTRACTION AND PURIFICATION OF LACCASE ENZYME FROM TERMITE GUTS

Abstract

           This study was conducted at the University of Baghdad, College of Agricultural Engineering Sciences, with the aim of extracting laccase enzyme from the bowels of termites. The concentration method was used by precipitation (with ammonium sulfate) with gradient saturation rates from 30% to 80%. The results showed that the highest effectiveness was at the saturation rate of 80%, reaching 5.991 units/mg, dialysis reaching 3.763 units/mg, and gel filtration reaching 7.644 units/mg. m and ion exchange amounted to 5.867 units/mg. The results of purification by gel filtration showed one distinct protein peak, and the ion exchange chromatography technique was used, as three protein peaks appeared, peak (1) representing the protein, and peak (2) representing the bound protein and it represents the laccase enzyme. After measuring the activity, one peak appeared, with an enzyme yield of 32.192. % and the number of purification times is 15,355.