ANTI- PYOCYANIN AND ANTI- HEMOLYSIN IMPACT OF SOME NATURAL AND COMMERCIAL PROBIOTICS AGAINST MULTIDRUG- RESISTANT Pseudomonas aeruginosa

التأثير المضاد للبيوسيانين والهيموليسين لبعض المعززات الحيوية الطبيعية والتجارية ضد عزلات الزائفة الزنجارية المقاومة للمضادات الحيوية

Abstract

The aim of this study is to evaluate the anti-pyocyanin and anti-hemolytic efficacies of lactobacilli probiotics isolated from natural and commercial sources against pathogenic Pseudomonas aeruginosa (P. aeruginosa).

Two isolates of P. aeruginosa (P50 and P78), which were determined as multidrug-resistant bacterial strains, were obtained from one hundred-thirty samples, including burn, wound, ear, sputum, and urine, collected from patients attending a number of hospitals in Baghdad, including Al-Karkh Hospital,  Al Kadimyia Teaching Hospital, and  Medical City Hospitals (Al-shahid Ghazi Al-Hariri Hospital for Surgical Specialties, Baghdad Teaching Hospital and Burns Specialized hospital) during the period of October and December 2022. The direct antibacterial effect of undiluted CFCS (cell-free culture supernatants) of Lactobacillus isolates and their anti-pyocyanin and anti-hemolytic were assessed.

The antibacterial effect of a natural isolate of Lactobacillus acidophilus (L. acidophilus) was higher than that of other isolates. The findings revealed that spontaneously isolated lactobacilli outperform commercially isolated lactobacilli regarding probiotic characteristics. All Lactobacillus isolates showed a strong anti-hemolytic and anti-pyocyanin effects against P. aeruginosa at 100 µg/ml, but this effect is not confirmed due to their antimicrobial activity against both strains of P. aeruginosa. Also, both isolates of Lactobacillus plantarum (L. plantarum) (natural and commercial) did not show an anti-hemolysin effect against both isolates of P. aeruginosa P50 and P78.

The findings supported using probiotics instead of antibiotics to treat antibiotic-resistant pathogenic bacteria and, consequently, various disorders linked with this pathogenic bacterium.