MOLECULAR DIAGNOSTICS OF Cuscuta sp AND ITS ASSOCIATED BACTERIA

Abstract

This study was conducted at the Faculty of Agricultural Engineering Sciences/ Plant Protection Department, University of Baghdad ـــ Jadriya, with the aim of isolating and diagnosing bacteria molecules from samples of Cuscuta sp.(stems and seeds) intruding on different plants, diagnosing Cuscuta sp. molecules and testing the effect of bacteria in the germination of Ocimum sp. and Cuscuta sp. seeds .The results of isolating and diagnosing bacteria from samples of Cuscuta sp.(stems and seeds) intruding on the plants Conocarpus, Chenopodium and Cynanchum showed the obtaining of three types of bacteria B1,B2 and B3 sequentially .The results of testing the efficiency of three types of bacteria that were isolated from the Cuscuta sp. (B1,B2 and B3) showed that the isolation of B2 bacteria had a significant effect in raising the germination rate of seeds of Cuscuta sp. and Ocimum sp. and did not differ significantly with the two comparative coefficients, while it differed significantly with isolates B1 and B3 .The results of the nucleotide sequence analysis of the two samples showed that Sample 1 belongs to the type Cuscuta reflexa and was recorded in the Gene bank under the serial number (OQ746922)and was recorded for the first time in Iraq .and the results also showed that the sample 6 belongs to Cuscuta campestris and was registered in the Gene bank under the serial number (OQ746927) .The results of the nucleotide sequence analysis of the isolates of bacteria B1,B2 and B3 showed that they belonged to Staphylococcus aureus, Bacillus subtilis and Achromobacter xylosoxidans sequentially and were deposited in the Gene bank under the accession numbers OQ976982, OQ976981 and OQ976979 On the relay .