ALPHA- LACTALBUMIN ISOLATION, PURIFICATION AND CHARACTERIZATION FROM IRAQI CAMEL MILK: ALPHA- LACTALBUMIN ISOLATION, PURIFICATION AND CHARACTERIZATION FROM IRAQI CAMEL MILK

Zaid A. Haddad; Kifah  Saed  Doosh

Authors

Zaid A. Haddad
Kifah Saed Doosh

Keywords:

Camel milk, α -lactalbumin, Isolation, Purification, Characterization.

Abstract

alpha-lactalbumin (α-Lac) is a crucial protein in several biological procedures like a strong antioxidant and prospective cancer therapy, This study was aimed to isolate, pacificate, and characterize α-lactalbumin α-Lac from Iraqi camel milk sourced from new local camel farms in Samawah Govermanate, southern Iraq. purified step done by ion exchange chromatography through DEAE-cellulose column and gel filtration chromatography through Sephadex G-100 column, the purification fold and yield were (58.20 time and 82%) respectively. The purity of α-Lac to homogeneity was examined by polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) with a single bond The molecular weight was determined by two methods and it was 14.40kDa and 14.23kDa, The quantitative and qualitative analysis using HPLC technique was without significant differences and the used of HPLC assay as an additional confirm purification step.

Author Biographies

Zaid A. Haddad

alpha-lactalbumin (α-Lac) is a crucial protein in several biological procedures like a strong antioxidant and prospective cancer therapy, This study was aimed to isolate, pacificate, and characterize α-lactalbumin α-Lac from Iraqi camel milk sourced from new local camel farms in Samawah Govermanate, southern Iraq. purified step done by ion exchange chromatography through DEAE-cellulose column and gel filtration chromatography through Sephadex G-100 column, the purification fold and yield were (58.20 time and 82%) respectively. The purity of α-Lac to homogeneity was examined by polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) with a single bond The molecular weight was determined by two methods and it was 14.40kDa and 14.23kDa, The quantitative and qualitative analysis using HPLC technique was without significant differences and the used of HPLC assay as an additional confirm purification step.
Kifah Saed Doosh

2Professor PhD., Department of Food Science., College of Agricultural Engineering Sciences, University of Baghdad, Baghdad, Iraq, [email protected] .

ALPHA- LACTALBUMIN ISOLATION, PURIFICATION AND CHARACTERIZATION FROM IRAQI CAMEL MILK