DETECTION OF ASPERGILLUS FUMIGATUS BY POLYMERASE CHAIN REACTION

Abstract

             This research was aimed to isolate and diagnose Aspergillus species from this -infected animals collected. From several Baghdad areas, Fifty samples were taken from animals with respiratory illnesses. Molds were isolated and identified using conventional methods (fungal culture on Czapek-Dox agar incubated at 37°C for 3–7 days, then macroscopic, microscopic examination of the colony with lactophenol cotton blue stain) and molecular methods (PCR for identification).  Partial ribosomal DNA (rDNA) from different Aspergillus strains was aligned to find the optimum primers for gene and species identification. A PCR approach for identifying Aspergillus fumigatus-related species was developed utilizing specific primers. To examine the specificities and sensitivities of such primers, 14 putative Aspergillus fumigatus isolates were PCR-tested. Only 3 were verified as having Glip P toxin. The PCR technique was sensitive and detected A. fumigatus isolates and closely related species. Our findings show that these primers can identify A. fumigatus. In a phylogenetic tree study, we compared our ITS sequence (Iraq), OR578448.1 to global sequences. Our sequence was more similar to Russia and Brazil, OR578448.1 and OR727320.1, but more different from Portugal, KF367498.1 and Pakistan, MT316338.1.  Detecting the gliP gene in A. fumigatus was especially fascinating because of its medicinal involvement in gliotoxin production. PCR detects Aspergillus fumigatus in aspergillosis quickly, precisely, and sensitively.