EFFECT OF PLANT PART TYPE AND GROWTH REGULATOR ON THE INITIATION AND MULTIPLICATION OF VEGETATIVE SHOOTS OF YELLOW PASSION FRUIT (PASSIFLORA EDULIS SIMS. F. FLAVICARPA) IN VITRO

Abstract

Experiments were conducted at the Plant Tissue Culture Laboratory - Postgraduate Studies Section of the Department of Horticulture and Landscape Engineering, College of Agricultural Engineering Sciences, University of Baghdad, during 2022–2024 using a Completely Randomized Design (CRD) Each treatment had 10 replicas in each experiment. The aim was to study the effect of plant part type and growth regulators on the initiation and multiplication of vegetative shoots of in vitro cultures of yellow passion fruit (Passiflora edulis Sims. f. flavicarpa), Initiation Stage involving two experiments: The first experiment examined two factors: plant parts and BA. The single node treatment combined with 4 mg L⁻¹ BA resulted in the highest number of shoots and the highest response percentage of plant parts, The second experiment examined two factors: plant parts and a combination of Kin + BA. The apical bud combined with 2 mg L⁻¹ Kin + 2 mg L⁻¹ BA showed significant superiority, providing the highest shoot number and plant part response percentage, For the multiplication stage, two experiments were conducted: The first experiment involved a single factor (BA), where the treatment of 2 mg L⁻¹ BA gave the highest number of shoots and shoot lengths, The second experiment involved a single factor (mT), where the treatment of 1 mg L⁻¹ mT achieved the highest shoot number and lengths.